FastQCFastQC Report
Thu 5 Jan 2017
ERR1629986_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1629986_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38556004
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCG26150.017.3982583
CGCCTTA49100.017.35241364
TGCGACG32500.017.30987410
CGTATCG33050.016.1828653
CCCGTAT44350.015.5880711
GCGTCTA106650.015.183351557
GACGTTA38050.014.96776813
TTGCGAC46650.014.6647449
CGTCTAG111900.014.53802158
GTATCGA34750.014.1917244
GTCGTTA55400.014.10409120
ACTACGC60900.014.09890647
CTCGTAT39950.014.087411
CAATCGG46700.013.9811414
GCTACCG42350.013.94119430
TCGACTA59100.013.8770744
TCGATTG42200.013.82512920
CGACGTT39900.013.75154512
GCGACGT41250.013.63820411
CCGTTAC31700.013.53051761
Produced by FastQC (version 0.11.5)