FastQCFastQC Report
Thu 5 Jan 2017
ERR1629985_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1629985_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39948381
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCC3058380.7655829656776328No Hit
AGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGC1317560.3298156188106847No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1205890.3018620454230674No Hit
CTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCC1115880.27933046898696595No Hit
AGCACATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGAC938140.2348380526359754No Hit
GGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGC707850.1771911607631859No Hit
GCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCC675440.1690781911787614No Hit
CAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG592500.14831639860448914No Hit
CTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAGACCCGCCGGG553470.13854629052426432No Hit
CCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCG518710.12984506180613428No Hit
AGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATGCGCC518080.12968735829369404No Hit
CACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCTCTGTTCTACAGCACACTACCAGA446050.11165659003802933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGC661700.035.5424922
ACAGGCT698900.033.5514263
GGACAGG703600.033.5294531
GTCGAAC101400.026.3366839
TCGAACG102800.025.94806940
ACGGTAA107500.024.78965444
TTGAACG116100.023.983737
AGGCTGC1011850.023.8545825
GCTGCAT984650.023.6798657
AGGACAG434850.023.3634381
GCCTAAC115600.023.23513824
AGTCGAA116500.023.21942538
GGCTGCA1028300.022.8949286
TGCATCA1023300.022.7917799
CGGTAAC118500.022.66664545
CGAACGG117700.022.63593141
AACGGTA118500.022.63334343
GAACGCT119300.022.616649
GCATCAG1046800.022.24158710
CAGGCTG1073500.022.1118474
Produced by FastQC (version 0.11.5)