FastQCFastQC Report
Thu 5 Jan 2017
ERR1629985_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1629985_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39948381
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGGGCTGCCTG727360.1820749631881202No Hit
CCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCA595140.1489772514185243No Hit
GAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGTTCACAA580100.1452123929628087No Hit
GTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAGGGCCAGCAGCGCCA526750.13185765901251417No Hit
CTCAAATTCATCGTGACGTTTGGCAATGTTATTCCTGTTCCTCTTGGTAT526710.1318476460910894No Hit
CACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAGGGCCAGCAGCGCCAGCA507260.12697886304829223No Hit
GTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGTTTGGCAATGTTATT501150.12544938930065777No Hit
GTAATTTTTGGCTACACAACACTAAAAGAAAATTTATTTATTGGCATGCA493450.12352190192638846No Hit
CCCAGCTCCACCTGCCCCACCTGCAGGTCCTCTGCCTCCCGGCGGGTCTT458350.11473556337614782No Hit
CCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTC454770.11383940690863042No Hit
CACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAGGGC447050.11190691307364872No Hit
CACGCTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCTGCA444840.11135369916492985No Hit
CTCTGCCTCCCGGCGGGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGC434540.10877537189805013No Hit
CCACGCTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCTGC420590.10528336555115964No Hit
CTTCAGCATGTCTCTCAAATTCATCGTGACGTTTGGCAATGTTATTCCTG417310.10446230599432803No Hit
CTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCAC404090.10115303546343968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACC78550.020.17740470
CGGTTCG38900.019.1945253
AACGCTC55050.018.64208463
AGAACGC56700.018.32893661
TTCGCAC92850.017.7643188
TGCGACG36000.016.3979810
CGCCTTA75200.016.02232264
TCTCTCG359700.015.68780813
GTAGTTC534500.015.41744970
TCTCGGT363550.015.29231615
CCCCTAC74400.015.23603769
TCGGTGC365200.015.12817717
ATCTAGC166550.014.88015755
GCTAAAT183850.014.7001011
TCGCACT73850.014.6234189
TTTATTC471050.014.6201322
CGGTGCA381250.014.61874918
CTTTATT566300.014.4889761
GCGTCTA380700.014.4533457
TATTCCA438250.014.3350484
Produced by FastQC (version 0.11.5)