FastQCFastQC Report
Thu 5 Jan 2017
ERR1629984_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1629984_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31291660
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCC1232100.3937470878822025No Hit
AGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGC634600.20280164107624846No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG632370.20208899112415254No Hit
CTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCC509990.1629795287306586No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTG389360.1244293207838766No Hit
CTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAGACCCGCCGGG329650.10534755906206317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAACG140650.034.258277
TCGAACG137700.033.93453640
GTCGAAC139350.033.72780239
ACGGTAA141000.033.37191844
GAACGCT143750.033.2107629
AACGCTG144850.033.0075210
CGGTAAC147900.031.81791945
GGCCTAA157950.030.46215423
AGTCGAA156900.030.30763238
AACGGTA155300.030.29782143
GCCTAAC159700.029.63032724
GAACGGT164400.028.72414242
CGAACGG165650.028.27460541
TGAACGC175200.027.7232938
GACAGGC332050.027.5872672
AAGTCGA175900.027.15062537
CAAGTCG180600.026.17165836
GGACAGG357400.025.5764541
ATTGAAC201950.025.385986
ACGCTGG197350.024.35046611
Produced by FastQC (version 0.11.5)