FastQCFastQC Report
Thu 5 Jan 2017
ERR1629984_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1629984_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31291660
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGGGCTGCCTG368800.1178588799699345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACC65500.026.05482170
CGCCTTA69300.025.51786664
AACGCTC49400.023.07884863
AGAACGC53900.021.00814661
TGCGACG52400.019.75458310
CGTATCG30550.019.5589373
CCCGTAT38250.019.350621
CCCCTAC67150.018.63221469
GACGTTA58250.018.48639313
GTATCGA31600.018.4690554
GTGTACG44150.018.28258966
ACGTTAT56300.018.26266514
CCGTTAC47500.018.19077761
TTCGCAC110100.018.109378
GCGACGT57950.017.80281611
TAGGGGT117050.017.57239769
TACGACG27400.017.49481414
ATCGTCG52000.017.4428444
GTACGGG47600.017.43937568
CGGTTCG51250.017.2851983
Produced by FastQC (version 0.11.5)