FastQCFastQC Report
Thu 5 Jan 2017
ERR1629983_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1629983_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33634267
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCC1532730.4557048916808563No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1046850.31124507633836646No Hit
AGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGC737650.21931502179012852No Hit
CTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCC593570.17647775704462357No Hit
CTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAGACCCGCCGGG421600.1253483538083348No Hit
CCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCG348500.10361456665608322No Hit
GGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGC345960.10285938444860417No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTG342280.10176526219524866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAACG132300.034.1455737
TCGAACG129700.033.93242340
GTCGAAC130300.033.82309339
ACGGTAA130450.033.7033744
GAACGCT132600.033.6027039
AACGCTG132800.033.58003610
CGGTAAC135450.032.54149645
AACGGTA143300.030.77300643
AGTCGAA146600.030.27192938
GACAGGC398250.030.1082362
GCCTAAC151950.029.57109824
CGAACGG151300.029.04624241
GGCCTAA156200.028.91994523
GGACAGG414500.028.7476061
GAACGGT154850.028.38444942
CAAGTCG156250.028.19533336
AAGTCGA169350.026.63229837
TGAACGC168850.026.6132688
ACAGGCT445950.026.60413
GTTTCGC59600.024.77783667