FastQCFastQC Report
Thu 5 Jan 2017
ERR1629983_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1629983_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33634267
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAGGGCCAGCAGCGCCA349760.10398918460152558No Hit
CCTCGTTCCCCGCACACTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCA345390.10268991442566595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTA75650.025.82940164
CCCTACC77700.025.24104770
AACGCTC58950.022.37545863
AGAACGC60250.022.09347361
CGGTTCG48600.021.2014883
TGCGACG43900.021.177310
CGTATCG28050.020.0367983
GAACGCT67300.019.74037762
GCGACGT48250.019.69951411
TAGGGGT114200.019.22916269
CCGTTAC44900.019.0640861
TTCGCAC110700.018.9583978
CCCCTAC76250.018.77345869
CCCGTAT36350.018.7119871
CGACGTT50600.018.3733312
AGGGGTC171000.018.20309370
TTGCGAC59150.018.1803999
ACGTTAT51700.017.91504114
CTATCGC17300.017.79717363
GCCGTTA47950.017.7660660
Produced by FastQC (version 0.11.5)