FastQCFastQC Report
Thu 5 Jan 2017
ERR1629982_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1629982_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28623261
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCC1092330.3816231840250487No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG767440.2681175984804806No Hit
AGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGC548350.19157495716508333No Hit
CTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCC444640.15534218829922977No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTG426540.1490186600331807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAACG151500.034.999057
GAACGCT159050.032.884169
AACGCTG163450.032.0003910
TCGAACG169550.030.72869540
GTCGAAC169900.030.68218239
GGCCTAA177150.030.03033323
GCCTAAC175350.030.02373724
ACGGTAA174250.029.83135844
CGGTAAC178400.029.14067645
AGTCGAA183800.028.49219338
TGAACGC186150.028.375128
GACAGGC301500.027.9469432
GAACGGT187150.027.91895542
AACGGTA189850.027.43304643
CAAGTCG192400.027.22991236
ATTGAAC210400.026.714876
AAGTCGA204150.026.0235937
CGAACGG201250.025.92596241
CGTGACG32250.025.67461870
GGACAGG331850.025.5724011
Produced by FastQC (version 0.11.5)