FastQCFastQC Report
Thu 5 Jan 2017
ERR1629982_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1629982_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28623261
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA296500.10358707905433975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACC78600.031.2510570
AGAACGC62700.026.19673761
CGCCTTA90100.025.96458464
AACGCTC63000.025.9257663
TAGGGGT107600.024.64054369
CCCCTAC78250.023.52966369
AGGGGTC161150.022.71877170
GAACGCT72500.022.56777262
CGGTTCG66600.022.5279883
CAGAACG82800.020.46053160
TTCGCAC139250.019.8010468
GTGTACG47900.019.35384266
TCGGTTC66400.019.1962412
CTTAGGG125150.019.14846467
ACCTTCG60350.018.94531445
ACGCTCC89100.018.65351364
TCGCACT88100.018.644559
GGCTTAC89750.018.61044754
CCCGTAT29050.018.5359731
GCCTTAG126850.018.50651465
Produced by FastQC (version 0.11.5)