FastQCFastQC Report
Thu 26 May 2016
ERR1357058_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357058_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856817
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAT3750.031.2796767
GCTCGAT3900.030.07660764
CGACGAG3600.029.7072224
CAATGCG4050.028.96109619
CTCGATA4100.028.60945565
CATTGCG4350.028.54757729
CGCGATA1900.027.23582314
TCGTCTA4300.026.48145954
CGTCATT4700.026.42311726
TGCGACG4150.025.7701222
GTCGTCT4900.025.35145253
AGGTCGT5250.024.97049322
GCTCGTC5000.024.83572439
TGGTCGT4600.024.75307351
ACCGTCG3100.024.48295423
TCGATAA4800.024.43724366
GCGATAT2150.024.06756815
GCGCGAT2200.023.52184713
ATCGTCA4700.023.4884810
GTCGTCA5450.023.4211424
Produced by FastQC (version 0.11.5)