FastQCFastQC Report
Thu 26 May 2016
ERR1357058_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357058_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856817
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAT3800.029.95680464
CTCGATA3850.029.56457365
TCGATAA4200.027.92209866
TTCGACG500.007749974327.58988625
CGATAAT4400.025.86830367
CGACGAG4250.025.15548324
TGGTCGT4600.024.7569351
TCGTCTA4750.024.69499854
GTCGTAC700.001319688224.6890071
CAATGCG4350.024.57719619
CTCGTAT17950.023.82413539
TATGCCG17450.023.71623643
CATTGCG4950.023.68828629
ATCGTCA4600.023.28034610
TCGTATG18300.022.99157340
GTCGTCT4950.022.99157153
GTACTAG6250.022.6743851
CGTCATT5300.022.12396426
GTCAGCG5750.021.59208543
GTCTACC5600.021.5644756
Produced by FastQC (version 0.11.5)