FastQCFastQC Report
Thu 26 May 2016
ERR1357052_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357052_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673227
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA5500.038.26201210
CGACGAG5450.037.98003824
CAATGCG5850.036.5625519
GCTCGAT7200.035.45682564
GTCAGCG7700.034.05050743
GTCGTCT7850.032.5286953
CTCGATA7750.032.4953965
TCAGCGC8650.031.10850544
TGGTCGT8100.031.09685151
TGCGACG6450.031.02193822
GTCTACC8300.030.34934256
TCGATAA8600.029.68478466
GCGCTGG8800.029.40216347
GTACTAG8500.029.2380221
CTCGACT605.425537E-428.74792127
GCTCGTC9400.028.25605839
CGGTCCG756.168203E-527.59800328
CGTCATT9650.027.52650626
GTCATCC7650.027.50863613
CGCCAGC10300.027.46156934
Produced by FastQC (version 0.11.5)