FastQCFastQC Report
Thu 26 May 2016
ERR1357052_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357052_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673227
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG6200.042.83489624
ATCGTCA6350.041.87435510
CAATGCG6550.041.07258219
GTACTAG8100.035.4097751
TGCGACG7700.034.49043722
GTCATCC8750.030.74759913
GCTCGAT8250.030.52336964
TAGGCAT12000.029.901545
ACGAGTC9000.029.89171226
CTCGATA8500.029.62385465
GTCGTCT8750.029.56323253
TGGTCGT8500.028.82516351
TCAGCGC9200.028.49210244
GTCCTAC9650.028.23569730
ATGCGAC10450.027.72437121
GTCAGCG9350.027.66612643
CCAATGC9900.027.52267318
GAGTCCT10300.027.12354728
CTAGGCA12900.027.0130214
GCGCTGG9400.026.79954347
Produced by FastQC (version 0.11.5)