FastQCFastQC Report
Thu 26 May 2016
ERR1357049_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357049_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320898
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTA150.002357667568.9728829
GCTCGTC2350.039.6227239
CGTCATT2300.038.99074626
AGGTCGT2600.038.4836422
TCGTCTA2250.038.35413754
CGTCTAC2300.037.5203555
TGGTCGT2300.037.5203551
TCGATAA2250.036.79700566
CAATTAG2550.036.5264417
GTCGTCT2400.035.95700553
CATTGCG2500.035.86589429
CTCGATA2350.035.22568565
GTCGTCA2550.035.17909624
GTCTACC2500.034.51872656
ATTAGGT2700.034.49719219
CGATAAT2450.033.7931767
TCGTCAG2900.033.2972541
CTGGTCG2600.033.1910850
CTAGTAC3250.032.940973
TAGTACT3050.032.8364524
Produced by FastQC (version 0.11.5)