FastQCFastQC Report
Thu 26 May 2016
ERR1357049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357049_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320898
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGT200.007357048351.74497655
TCGTCTA1450.035.68062654
GTTACAC400.002596777234.4912723
GTCGTCT1600.032.3305353
AGCGTAT751.6433551E-632.252187
CGCATTG1207.8216544E-1131.61207214
CGTGACC553.2418044E-431.38015750
CTAACTG553.256664E-431.3557034
ACTCGAG901.9947402E-730.65412938
TATCGCA450.00462702330.65412922
AATACTA450.00462702330.65412918
GTGTAGG1700.030.4952641
TCGATAA1700.030.43347566
CTCGATA1600.030.17986565
CGATAAT1600.030.17986567
TGGTCGT1850.029.85355451
TATCTTG1052.329216E-829.5593439
GCTCGAT1650.029.26532264
ATCGTCA953.1467061E-729.09970710
GAGCGTA953.1467061E-729.0997076
Produced by FastQC (version 0.11.5)