FastQCFastQC Report
Thu 26 May 2016
ERR1357048_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357048_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2384614
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG6400.038.8120324
CAATGCG7050.036.2085319
ATCGTCA7100.034.98402410
GTACTAG9700.029.9022711
TCGATAA9650.027.88333166
GCTCGAT9700.027.73902164
CTCGATA9850.027.31659965
TCGTCTA10250.027.27338254
TGCGACG9200.026.62467622
CGATAAT10500.026.28318867
TGGTCGT10400.026.21356251
ACCGTCG6050.025.66084523
GCTCGTC11500.025.1938839
GACCGTC6250.024.83969722
GTCGTCT11000.024.78633153
AATCGCG700.00133517724.63997320
GTCTACC11300.024.4337156
ACGAGTC10050.024.37079226
CTGGTCG11650.024.2895950
TAGGCAT12500.024.024815
Produced by FastQC (version 0.11.5)