FastQCFastQC Report
Thu 26 May 2016
ERR1357048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357048_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2384614
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA58030.2433517542042444No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA6500.038.27198410
CGACGAG6650.037.85908524
CAATGCG7200.034.9670719
TCGATAA9900.033.4443866
GCTCGAT10600.031.23775164
CGATAAT10600.030.91041467
TGGTCGT10900.030.71182451
CTCGATA10700.030.62217365
TCGTCTA11000.030.42113754
GTCGTCT11300.030.2152153
CTGGTCG12100.028.52167350
GTACTAG9850.028.4179521
TCAGCGC12400.027.8129644
GTCAGCG12700.027.15596243
GTCTACC12400.026.98819256
TGCGACG9250.026.84476722
GCTCGTC12950.026.63171439
CATTGCG12800.026.1354929
GCGCTGG13500.025.5638747
CGTCATT13650.025.26598426
Produced by FastQC (version 0.11.5)