FastQCFastQC Report
Thu 26 May 2016
ERR1357047_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357047_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1552149
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA5150.038.1779610
TCAAGAC1300.037.187053
CAATGCG5400.036.41163319
CGACGAG5450.036.07874324
GAGTCCT5600.033.87815528
TGGTCGT6650.033.2101951
CGGTCCA751.6669164E-632.20696351
GCGATGA553.2608068E-431.3582929
CCGCATG553.2626555E-431.3552633
GTCATCC6400.030.72231513
AACCGTG450.00462266830.6673687
TGCGGGC450.004626312830.66242414
CCCGTAG450.00462704230.66143834
CGTAGCG450.00462704230.66143836
TACCCCG703.8352096E-529.5902235
GCAAGAA2500.028.97599418
ATGCGTC2150.028.8825268
GCTCGTC7750.028.48546439
TGCGCAT854.43534E-628.40692134
GTCAGCG7800.027.86422543
Produced by FastQC (version 0.11.5)