FastQCFastQC Report
Thu 26 May 2016
ERR1357047_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357047_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1552149
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTGC605.6243152E-945.98962418
ATCGTCA5500.039.57812510
CGACGAG5750.038.98869324
CAATGCG5750.038.98869319
CCCGGCG901.200533E-1038.391488
TCAAGAC1900.038.1266673
TGGTCGT5700.037.53115551
CGGTCCG655.465263E-737.14307428
CGGCGCT952.1645974E-1036.37087610
TACCCCG952.2009772E-1036.311115
GTCAGCG6150.035.3312243
GAGTCCT6400.035.02890428
GCTCGTC6650.032.67473239
GTACACG553.2317807E-431.406141
GTCGTCT7000.031.04099853
GCGCTGG6900.031.00399847
GCTCGAT7050.030.82283264
TAGCGAT450.004589031530.7131827
ACGTTGG901.9937033E-730.6706250
AGCGACC450.004628287630.65975217
Produced by FastQC (version 0.11.5)