FastQCFastQC Report
Thu 26 May 2016
ERR1357045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357045_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041686
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA41780.4010805559448816RNA PCR Primer, Index 31 (96% over 29bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG21290.2043802067033636No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG20670.19842831717043333No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC14370.13794943965839992No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCT13910.13353352161783877No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT13260.12729363743008928No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT13190.12662164990217784No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA12750.1223977282981628No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA12570.12066976036924754No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCACAAGCACGATCGGCTCCTAAT12520.1201897692778822No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA12130.11644583876523251No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT12080.11596584767386718No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG12030.11548585658250182No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11950.11471787083631728No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC11660.11193392250639829No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTG11370.10914997417647929No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTT11090.10646202406483336No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA11040.105982032973468RNA PCR Primer, Index 31 (96% over 29bp)
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA10930.10492605257246426No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAAT10450.10031813809535696No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGT150.002347907769.0560767
GACTGCC200.00736594851.73736630
CGGCGCT404.9777766E-543.1600510
CCCGGCG503.821053E-641.4336478
CGGCGAT350.00134937939.41894532
CATATAG350.00134937939.41894521
ATTCAAG1750.037.5362851
TCAAGAC1850.037.2971423
GTACCCA753.9461156E-836.877751
ATCGTCA3900.034.52803810
TACCCCG601.3424649E-534.499865
CTAACCA501.8639721E-434.49157338
CTAACTG2450.033.7957764
CGACGAG3950.033.1817724
GAGTCCT4000.032.76699428
GACGAAG851.2108285E-732.4626626
CAATGCG4000.031.90470719
TCAATAC450.004619332530.67095649
CTTTATG450.004619332530.67095648
ACCGAAT901.9991603E-730.65917814
Produced by FastQC (version 0.11.5)