FastQCFastQC Report
Thu 26 May 2016
ERR1357044_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357044_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978329
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGG1305.456968E-1231.84292227
ACCGTCG1750.031.54126723
CGACGAG2300.031.49841124
CGTAGAC553.243989E-431.3845163
CAATGCG2300.029.99695219
TAACGGA1401.4551915E-1129.56691628
ATGCGTC756.162554E-527.60002168
CCGTCGG1900.027.2354724
GTCGACG2050.026.93373350
TTCGGAC1306.2882464E-926.54526550
CGTAAGC1306.3118932E-926.53576961
TCGACGA2100.026.29245451
TGGTCGT2500.026.22672551
CCGTAAG1457.039489E-1026.16975860
GCTAACG1457.039489E-1026.16975826
CGTGGTT2000.025.8710536
GACCGTC2150.025.67312622
ATTCGGA1359.418727E-925.5568849
TGCGACG2900.024.981522
TATGACG700.001333698924.64287666
Produced by FastQC (version 0.11.5)