FastQCFastQC Report
Thu 26 May 2016
ERR1357044_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357044_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978329
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG1900.032.673924
CTCGATA2850.031.46375365
CGATAAT2900.030.92127667
TCGATAA3050.029.40055566
GCTCGAT3100.028.92783264
TGGTCGT3050.028.2842251
TGCGACG2200.028.21836722
CGTAACG500.00774628527.59129123
ATCGTCA2300.027.04400310
CAATGCG2400.025.86683519
GTCGTCT3350.025.73814453
GCTCGTC3500.025.62048539
GTCTACC3400.025.36093956
CTGGTCG3650.024.5801550
GGTATCA27300.024.4336131
GCGCTGG3700.024.24798447
CGACTAT2300.023.99242836
GTCCTAT2450.023.9814741
GTCAGCG3900.023.87707943
CGGGATT750.001990545323.004545
Produced by FastQC (version 0.11.5)