FastQCFastQC Report
Thu 26 May 2016
ERR1357042_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357042_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1871847
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG5550.037.2925619
CGACGAG5650.035.41332224
TGGTCGT8650.033.9101251
GCTCGAT8600.033.2940664
GTCAGCG8850.033.13581543
ATCGTCA6250.033.1175610
CTGGTCG8800.032.93995750
GCTCGTC9050.032.39660339
GTCGTCT9100.031.8557353
TCGTCTA9650.030.75535854
GCGCTGG9650.030.74713947
GTCTACC9750.030.43910456
CTCGATA9450.030.30016765
CATTGCG9700.029.869329
TCGATAA9750.029.36863966
CGTCATT10200.029.08376326
CGATAAT9850.029.0704867
TCAGCGC10200.028.75018744
GCGTTCG605.430914E-428.74327735
GTCGTCA10550.027.46717524
Produced by FastQC (version 0.11.5)