FastQCFastQC Report
Thu 26 May 2016
ERR1357042_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357042_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1871847
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG5850.036.55232619
CGACGAG5950.035.35835624
CTGGTCG9550.035.0502650
GTCGTCT9350.035.04218753
TGGTCGT9750.033.9773551
GTCAGCG9950.032.9290943
ATCGTCA6400.032.92675410
GTCTACC9750.032.9085256
TCGTCTA10100.032.4487154
GCGCTGG10150.032.2983647
CTCGATA9800.031.67685365
CGATAAT9800.031.32405367
GCTCGTC10250.031.29235639
TCAGCGC10600.031.2352244
GCTCGAT10000.031.04414464
CGTCATT11400.028.7407426
TCGATAA10850.028.61058666
CATTGCG11600.028.2452129
CGCCAGC12000.028.16592434
GTCGTCA11650.028.12398524
Produced by FastQC (version 0.11.5)