FastQCFastQC Report
Thu 26 May 2016
ERR1357035_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357035_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1732916
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA6850.037.26871510
CGACGAG6800.037.03434824
CAATGCG7250.035.2104819
CTCGATA9300.032.6420965
TGGTCGT9700.031.65988551
GCTCGAT9400.031.5608664
CTGGTCG9750.031.49752650
TGCGACG8200.030.7114122
GCTCGTC10250.030.62373439
TCGTCTA10150.030.25973954
GTCGTCT10450.029.72127553
CGATAAT10150.029.56864467
GTCAGCG10800.029.3869743
TCGATAA10300.029.13803366
GCGCTGG11500.027.8997947
GTACTAG10150.027.8926471
ATGCGAC9450.027.7442221
GTCTACC11350.027.66857156
TAGGCAT11850.027.0890395
CGTCATT11900.026.6697626
Produced by FastQC (version 0.11.5)