FastQCFastQC Report
Thu 26 May 2016
ERR1357035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357035_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1732916
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGT9800.039.4431951
CTGGTCG10100.038.6133250
GCTCGAT10250.037.35541564
CTCGATA10250.037.35325665
TCGTCTA10550.035.97347654
GTCGTCT10550.035.95894653
CGATAAT10650.035.30256367
GTCAGCG11150.034.6425543
GCTCGTC11450.034.0360939
CGACGAG7500.034.02805724
TCAGCGC11500.033.5882144
GTCTACC11300.033.58682356
CATTGCG11700.033.30882329
TCGATAA11400.032.98002666
CAATGCG7800.032.27713419
GCGCTGG11900.032.19260447
ATCGTCA7950.032.15296610
CGTCATT12150.031.79131126
CGTCTAC12400.030.6073555
CTGTGCG13350.030.0146439
Produced by FastQC (version 0.11.5)