FastQCFastQC Report
Thu 26 May 2016
ERR1357030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251143
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG5500.031.9891824
GCTCGAT7450.031.95128864
TGGTCGT7300.031.67111451
GTCGTCT7600.031.32972353
ATCGTCA5850.030.66364510
CAATGCG6000.030.4733519
GTCAGCG8250.029.27244643
TCGTCTA7900.029.26636554
CTGGTCG8000.028.89989350
CTCGATA8300.028.68044565
CGATAAT8150.027.93838167
TGCGACG6550.027.38783322
GCTCGTC8950.026.97878539
CGTCATT8850.026.50409726
GCGCTGG9300.025.59652747
CGTCTAC9400.025.33041455
TCAGCGC9850.024.86777944
GTCTACC10050.024.72222356
ACCGTCG5600.024.64152323
TCGATAA9450.024.46008166
Produced by FastQC (version 0.11.5)