FastQCFastQC Report
Thu 26 May 2016
ERR1357030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251143
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG6350.039.64743824
GCTCGAT7400.038.69090364
TGGTCGT7400.037.77453651
CTCGATA8100.035.34488765
CGATAAT8100.035.34488767
CAATGCG7050.035.22162219
TGCGACG7300.034.96027822
GTCAGCG8650.033.88978643
GTCGTCT8750.032.7141853
TCAGCGC9250.032.0643744
TCGTCTA9400.031.19620754
CTGGTCG9100.031.09698550
ATCGTCA7750.030.75859310
TATAGCG450.00462657230.662675
CGTCTAC9700.029.87637755
TCGATAA9850.029.41552266
GTACTAG11850.029.1690961
GCTCGTC10050.029.16882139
CGTCATT10250.028.26320626
GTCTACC10250.027.93666856
Produced by FastQC (version 0.11.5)