FastQCFastQC Report
Thu 26 May 2016
ERR1357029_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357029_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514479
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG62820.4147961114020069No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC37220.24576108351452874No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG36540.24127109058626764No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG29980.19795586469010135No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT28290.18679691167721704No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT27890.1841557393664752No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCT27490.18151456705573335No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT24660.16282827295723482No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA24590.16236606780285498No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA24070.15893254379889057No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA24040.15873445587558493No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC23840.157413869720214No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22230.1467831511694781No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA22130.14612285809179262No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTG22010.14533050639857006No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCACAAGCACGATCGGCTCCTAAT21750.14361374439658786No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAG21350.14097257208584602No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACATTCAAATGAGCATCCTAGTCACCGCG21290.14057639623923474No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTG20540.1356241981565938No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA20490.13529405161775107No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTG20490.13529405161775107No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAGACATAACGATAGCGACTCT19250.12710641745445134No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGAT19200.1267762709156086No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGG18880.12466333306701512No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAAC18450.12182407283296764No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTT18100.11951304706106852No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCA17650.11654172821148395No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAAT17610.11627761098040976No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA17360.1146268782861961No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17280.11409864382404775No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTGCTAGCCCGTC17260.11396658520851065No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGAC17110.11297614559198246No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCC17040.11251394043760264No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTACTA16710.1103349732812406No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCT16560.10934453366471242No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTA16560.10934453366471242No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCT16130.10650527343066493No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTG16000.10564689242967384No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAA15770.10412821835099728No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA15710.10373204250438599No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGG15660.10340189596554326No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG15480.10221336842570942No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTG15240.10062866503926433No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCA15200.10036454780819014No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGATC15190.1002985185004216No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG15170.10016645988488451No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGG150.002346173469.0704810
GTGACGT150.002346173469.070489
CGCCGAG150.002355105269.00432659
GTACCCA252.5453646E-455.2966041
GTACTTA200.007331751351.7994356
AAAGGTC200.00735381651.76008250
TCGCCGA200.00735765951.75324258
TTTCGTA200.007360541351.748112
CTGGTCC200.00736438651.7412866
CGGCGAG200.00736727151.73615633
TAAAGCA200.00736727151.73615636
AACGGTC200.00736727151.73615652
TTAGTCA200.00736727151.73615640
CCCGACG200.00736727151.73615644
CTACACC605.6261342E-945.98769833
AGTCCTA8100.044.7102629
CGACGAG8200.044.16501624
CAATGCG8300.044.0484619
GAGTCCT8600.042.11082528
ATCGTCA9000.040.67483510
Produced by FastQC (version 0.11.5)