FastQCFastQC Report
Thu 26 May 2016
ERR1357028_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357028_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214090
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCAA400.00259415734.50605858
ACGCTAA400.002597829334.49610513
TCGATAA6800.033.48565366
AGTCCTA5050.033.4700929
GCTCGAT6700.033.4677564
GTCTACC7500.032.20963756
CGACGAG5300.031.24433324
CAATGCG5550.031.07885219
TCGTCTA7250.030.94156654
TTGCACG450.004627209630.66067731
CGTAGAC1251.2369128E-1030.3791073
CTGGTCG7300.030.25313450
GTCAGCG7950.029.9437243
CTCGATA7500.029.8990965
TCAGCGC8100.029.81513444
ATCGTCA5450.029.7501610
CGATAAT7700.029.57174567
CGTCTAC8000.029.33498855
TGGTCGT7650.029.32008251
GTCGTCT7850.029.01624953
Produced by FastQC (version 0.11.5)