FastQCFastQC Report
Thu 26 May 2016
ERR1357028_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357028_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214090
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTA306.321444E-445.98544713
CGACGAG5700.038.72299224
TGGTCGT8050.037.73032451
CTGGTCG8050.037.3015750
GCGCTGG8200.036.6192347
CGTCATT8500.035.2991426
CAATGCG6300.035.03508819
GTCTACC8500.034.91065656
TCGTCTA8500.034.9077854
GCTCGAT8100.034.49476664
GTCGTCA8700.034.48766324
TCGATAA8300.034.0763666
GCTCGTC8850.033.90312639
TCAGCGC8900.033.3251644
CGATAAT8400.033.26007567
CGCCAGC9050.033.1538934
AGGTCGT9050.033.1538922
GTCAGCG8950.033.13898543
CGCTTAA1154.7293724E-1132.988219
CTCGATA8500.032.8687865
Produced by FastQC (version 0.11.5)