FastQCFastQC Report
Thu 26 May 2016
ERR1357026_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357026_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354943
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG4150.034.08417524
ATCGTCA4250.032.46803710
TCGCGAG450.00462642930.66199140
CAATGCG4700.030.09337619
TGCGACG5150.027.46588722
TGGTCGT5300.027.34554951
ACCGTCG4000.026.7373723
GCTCGAT5450.026.58702964
GACCGTC4200.026.2855922
GTACTAG5800.026.1858231
CCGTCGG4150.025.7709624
CTGGTCG5650.025.65157750
GCATCGT5450.025.3219138
TCGTCTA6050.023.95560654
GCGCTGG6650.023.8671947
GTCAGCG6400.023.7177643
ATGCGAC6550.023.70217521
GTCGTCT6150.023.56608253
CTCGATA6300.023.54750665
CGATAAT6200.023.37085367
Produced by FastQC (version 0.11.5)