FastQCFastQC Report
Thu 26 May 2016
ERR1357026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357026_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354943
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTG309.059493E-657.5719510
TATAGCG200.00736054551.747812
GTCGAAC200.007369143451.73253627
ATCGTCA4200.039.47791310
CGACGAG4200.039.41526424
TATTCGC459.943103E-538.38139
GCTCGAT6750.036.28467664
CAATGCG4700.035.95594419
ACGTAAT709.738342E-734.49981756
CTCGATA7500.032.65379765
TCGATAA7800.032.2823366
GCGTTAT751.6739305E-632.18913352
CGATAAT7750.032.04434667
GTCAGCG8050.031.70358343
GTACTAG6000.031.6797961
TCAGCGC8150.031.31457944
TGGTCGT8000.031.06015451
ACCGTCG4800.030.89581923
TGCGACG5400.030.65631922
TCGTCTA8250.030.10781754
Produced by FastQC (version 0.11.5)