FastQCFastQC Report
Thu 26 May 2016
ERR1357025_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357025_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443206
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG4500.035.2661524
CAATGCG5050.032.78819319
ATCGTCA4850.032.72117610
GCTCGAT6100.032.2361264
CGATAAT6050.031.93231267
TCGTCTA6150.031.42071254
CCGTTCG450.004628898630.6587832
GTCGTCT6450.030.49426853
CTCGATA6650.029.56997365
GTACTAG6650.028.5551951
GTCAGCG6700.028.31852143
GCTCGTC7250.028.06961439
TCGATAA7000.027.5986466
GTCTACC7200.027.31778356
TTCGCGT1404.6929927E-1027.10205330
TGGTCGT7300.026.47087551
TCAGCGC7350.026.28351244
CGTCATT7900.026.20124226
GGTATCA26750.026.2009071
CATTGCG8050.026.13794729
Produced by FastQC (version 0.11.5)