FastQCFastQC Report
Thu 26 May 2016
ERR1357025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443206
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACC7650.036.5312856
CGACGAG4250.036.5173624
GCTCGAT7400.036.35916564
CGATAAT7600.034.94483667
TCGTCTA8050.034.71365754
TGGTCGT7800.034.5101451
CTGGTCG7850.034.2903350
GTCGTCT8150.034.2770353
CTCGATA7750.034.2708665
GTCAGCG8050.032.98911343
TCGATAA8300.031.99879366
GCGCTGG8550.031.88656247
CAATGCG5150.031.4750519
GCTCGTC9150.030.15398239
TCAGCGC8900.029.83846944
CGTCATT9250.029.82799326
CGTCTAC9600.029.11086855
CGCCAGC9600.028.74051734
ATCGTCA5600.027.76119610
GTACTAG7000.027.6358931
Produced by FastQC (version 0.11.5)