FastQCFastQC Report
Thu 26 May 2016
ERR1357024_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357024_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071521
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG1400.034.4973624
CAATGCG2000.029.32275819
CTCGATA2250.029.13246765
CCGAAAT906.909646E-626.83128460
TGGTCGT2550.025.70751651
ACGTCGT951.051304E-525.41910723
CGATAAT2850.024.20980567
GTGTACG1001.5554324E-524.1662041
ATCGTCA2200.023.52202810
CGGGATA1953.8198777E-1123.00146350
ACGTGCG1052.2811168E-522.9993139
ATGCGAC2400.022.99824321
TACGACG750.001994903722.99609427
TGCGACG2300.022.49828122
CCTACAC5550.022.3933933
GTACTAG3550.022.367111
GGTATCA21650.022.1649761
CGCGATA1103.268571E-521.95286614
GTATCAA31200.021.908921
ACCGTCG1906.7484507E-1021.78780723
Produced by FastQC (version 0.11.5)