FastQCFastQC Report
Thu 26 May 2016
ERR1357024_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357024_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071521
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG1550.033.37614423
GCTCGAT2800.033.2647164
CGATAAT2900.032.1146567
CTCGATA2850.031.47070365
CGCGATA1450.030.9208914
CCGTCGG1850.029.8280524
TGGTCGT3400.029.433351
GTCAGCG3200.029.09982943
CATTGCG3550.028.17385529
GCTCGTC3450.027.99081839
CGGGCGT2000.027.634796
GCGAGAC500.007747079727.59094829
GTCTACC3650.027.412256
ACGTGCG1653.6379788E-1227.2160849
GCGATAT1653.6379788E-1227.17290315
CGTACGA1800.026.8345560
TCGATAA3350.026.77233566
TCGTCTA3900.026.53718654
CGTCATT3750.025.7515526
GCGCGAT1759.094947E-1225.62016713
Produced by FastQC (version 0.11.5)