FastQCFastQC Report
Thu 26 May 2016
ERR1357021_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357021_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135368
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAT3700.027.03785167
TGCGACG4050.025.55361422
CTCGATA4000.025.00942665
ATGCGAC4100.024.40058521
CAATGCG4100.024.39886919
TCGATAA4200.023.818566
GCTCGAT4550.023.50260764
TGGTCGT4700.022.02579351
TCGTCTA4950.021.6125254
GTACATG73900.021.3537981
AATGCGA4550.021.2281620
GTACTAG6450.020.8789331
TACATGG73150.020.8174552
CTAACGC2553.6379788E-1220.3120823
ACTACGC850.00411978220.2954278
CGCACTA4300.020.06084458
ACATGGG73150.020.0149693
ACGAGTC5000.020.0084826
CGCGATA2252.582965E-1019.93041814
CGTCTAC5250.019.72017955
Produced by FastQC (version 0.11.5)