FastQCFastQC Report
Thu 26 May 2016
ERR1357021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357021_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135368
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA1502.8981958E-822.99390414
CGATAAT3450.022.99390467
TCGACGA2005.2750693E-1122.43323951
CAATGCG3250.022.28483219
ACCGTAT953.0384265E-421.8194668
CGACGAG3050.021.48459824
CTCGATA3700.021.44026265
GTCGACG2101.0186341E-1021.36499250
TGCGACG3300.019.85697722
TCGATAA4000.019.83224166
CGCACTA3000.019.55168758
CGCGTTA2153.1377567E-919.2628947
CGACGAA2651.1641532E-1018.22030452
GTACATG74550.018.1666661
TACATGG73500.018.1621932
CGACTAT3051.8189894E-1218.09229336
CATGGGG44550.018.0406784
AGTCGAC2501.0459189E-917.94659249
ACGTGCG2156.4967026E-817.6754599
ACATGGG73850.017.6557433
Produced by FastQC (version 0.11.5)