FastQCFastQC Report
Thu 26 May 2016
ERR1357018_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357018_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663399
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG1350.035.77578422
GCGCACT1305.456968E-1231.84918257
CTAACGC553.240808E-431.3879223
CGACGAG1550.031.15955424
CGCACTA1359.094947E-1230.66958258
ATACGTC450.00462767330.65802436
CAATGCG1700.030.43718519
ACGAGTC1650.029.26888826
GTATTCG500.007734700627.59846324
ATGCGAC1750.027.59846321
GCACTAC1653.6379788E-1227.18030459
GTCGTCT1155.6099452E-827.01071453
GTACTAG2450.026.775821
AATGCGA1950.026.5349820
GCTCGTC1306.315531E-926.53098139
GCGACGA1850.026.10665123
ATCGTCA2000.025.87355810
GTCCTAC1901.8189894E-1225.41580230
GCATCGT2200.025.0932948
CTCGATA1101.1528664E-625.08761865
Produced by FastQC (version 0.11.5)