FastQCFastQC Report
Thu 26 May 2016
ERR1357018_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357018_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663399
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG1051.6370905E-1136.1329524
CAATGCG1154.7293724E-1132.9909519
TGCGACG1207.8216544E-1131.61632722
CTGGTCG1151.6934791E-930.01213650
TGGTCGT1052.3186658E-829.58338751
GCGACGA1301.9826984E-1029.18430323
ATGCGAC1301.9826984E-1029.18430321
GGTATCA13450.029.0099811
CGATCGG605.421284E-428.74861761
AATGCGA1452.1827873E-1128.54389620
CTCGATA1103.668174E-828.21953265
AACGCGA500.007742953527.59243241
CGATAAT1005.02434E-727.59243267
ACGATCG658.648029E-426.54118560
TATGCGC809.529616E-525.88546450
GTCAGCG1208.529423E-825.86790543
TCTAACG809.573688E-525.8679052
GTATCAA21450.025.756331
ATCGTCA1501.02591E-925.32933610
TCGATAA1251.2661985E-724.83318766
Produced by FastQC (version 0.11.5)