FastQCFastQC Report
Thu 26 May 2016
ERR1357017_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357017_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980634
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATA6700.028.319965
CGACGAG5000.028.2882924
GCTCGAT7000.027.59833364
CGATAAT7500.026.21907867
CAATGCG5750.025.7977919
TGCGACG5700.024.8142922
TCGTCTA8200.023.56729154
ATCGTCA6050.023.37875410
TCGATAA8500.023.1344866
TGGTCGT8600.022.87067451
GTACTAG7500.022.0937351
TCTAACG3650.021.7532482
GTCTACC9400.021.6600656
GCTCGTC9250.021.6289439
CGTCTAC9300.021.15083155
TCAGCGC9500.021.06241844
AATGCGA7700.021.05663920
GGTATCA50200.021.0429611
ATGCGAC7750.020.92131621
CATTGCG9450.020.80616429
Produced by FastQC (version 0.11.5)