FastQCFastQC Report
Thu 26 May 2016
ERR1357017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357017_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980634
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG5150.034.15192424
CTCGATA7900.030.99989365
CGATAAT7900.030.9983367
GCTCGAT8050.030.8515264
TGCGACG5900.030.395122
GTACTAG6950.030.3309651
CAATGCG5950.030.13967919
GTCTACC8750.028.7848856
ATCGTCA6350.028.2911110
ATGCGAC7250.028.06507521
TGGTCGT9000.027.612451
TCGTCTA9050.027.44736354
GTCGTCT9050.027.4369753
GCTCGTC9500.026.86336139
TCGATAA9250.026.47558866
CTGGTCG9600.025.88662350
AGGTCGT9850.025.55870622
TCAGCGC10050.025.39322744
GCGCTGG10100.025.28858447
GTCAGCG9900.025.08126843
Produced by FastQC (version 0.11.5)