FastQCFastQC Report
Thu 26 May 2016
ERR1357015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588598
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG5600.036.34794619
CGACGAG5950.034.7896624
CACGCGT400.002597126434.4986616
ATCGTCA6400.032.34249510
ACCGTCG5550.031.08085423
GTCCTAC7000.029.07378230
CCGTCGG5950.028.99138524
AGTCCTA7250.028.54702229
GCTCGTC8300.028.26040639
CTGGTCG8100.028.11533250
GTCAGCG8350.028.09383443
TCGATAA8500.027.59806366
GTCGTCT8150.027.52033453
TGGTCGT8150.027.51946851
TGCGACG7400.027.50655622
CGTGGTT6150.027.48327836
TTACTAG6200.027.26421443
ATCGTGG6350.027.1591734
TCGTGGT6450.026.73978235
GTCTACC8700.026.57379356
Produced by FastQC (version 0.11.5)