FastQCFastQC Report
Thu 26 May 2016
ERR1357015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588598
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGTCG7350.033.7993950
CGATAAT7400.033.561567
TGGTCGT7550.032.9040451
TCGATAA7550.032.89472266
GTCAGCG7800.032.72382743
CAATGCG5150.032.14851819
CTCGATA7900.031.4373665
CGACGAG5050.031.41907724
GTCGTCT7800.031.39718653
GCGCTGG8250.030.53040947
GCTCGAT8250.030.10365364
AGGTCGT8900.029.84198422
TCGTCTA8250.029.6883554
GTCTACC8500.029.22100856
CATTGCG8700.028.94213529
GCTCGTC8850.028.84133739
ATCGTCA5400.028.13880510
CGTCATT9400.027.88770126
TCAGCGC9250.027.59414744
ATTAGGT9850.027.31400119
Produced by FastQC (version 0.11.5)