FastQCFastQC Report
Thu 26 May 2016
ERR1357014_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357014_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291809
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG5600.043.12439324
CAATGCG5900.040.92846319
ATCGTCA6450.036.9064610
TGGTCGT8150.035.14536351
CGCTCCG400.0025946634.5048649
AGGATCG400.002595645834.5021868
GAGTCCT7400.033.559328
GTCAGCG9000.032.96365443
CTGGTCG8950.032.77505550
GTCTACC9250.032.0864156
GTCATCC7550.031.98259213
GCTCGTC9600.031.61843539
GCGCTGG9150.031.29464147
ACGCTCC450.00462321730.66623748
TGCGACG7900.030.56919122
TAGGCAT10000.030.372515
AGTCCTA8050.029.99145129
CTCGATA9550.029.98155265
GCTCGAT9550.029.98155264
GTACTAG8550.029.8846861
Produced by FastQC (version 0.11.5)