FastQCFastQC Report
Thu 26 May 2016
ERR1357014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357014_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291809
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTT350.001349933839.41636330
CAATGCG6600.038.6698419
CGACGAG6800.036.01090624
ATCGTCA7400.034.55083510
CGAACGA400.002600399534.48931553
TGGTCGT9150.034.31944751
CGAGTGA851.2111013E-732.46304765
TCGAGTG851.2111013E-732.46304764
CTGGTCG10000.032.43753450
GTCAGCG10100.032.0989743
CGGCGAT652.3266637E-531.83629432
GTCTACC9650.030.74016456
GCTCGAT9650.030.73897464
GCTCGTC10750.030.47893139
GCGCTGG10700.030.31545447
GTACTAG8850.030.071671
GTCATCC8750.029.95991313
GTCGTCT10650.029.46974453
GACGACG2000.029.31818854
TCAGCGC11200.029.25433244
Produced by FastQC (version 0.11.5)