FastQCFastQC Report
Thu 26 May 2016
ERR1357012_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357012_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627012
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG4500.041.3991924
ATCGTCA5200.036.4896710
TGCGACG5450.033.549822
CAATGCG5700.033.28779619
GTACTAG7000.031.060851
CTCGATA5450.029.11869465
CGCACTA6050.028.51183758
ATGCGAC6800.028.41120721
CGATAAT5500.028.22671967
ACGAGTC6500.027.5994626
TCGACGT500.00773658827.59945926
GCATCGT7000.027.108288
AATGCGA7100.026.72482920
GGTATCA24950.026.4200521
CTGGTCG5750.026.40191750
TGGTCGT5900.026.31547251
GCTCGAT5900.026.31304664
GCGCACT6850.026.18926857
GCGACGA7000.026.12091823
GACGAGT7000.026.12091825
Produced by FastQC (version 0.11.5)