FastQCFastQC Report
Thu 26 May 2016
ERR1357012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357012_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627012
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG4250.038.14352824
CAATGCG4550.037.1446819
CTCGATA5600.033.26169665
CGATAAT5500.033.23929667
TGCGACG5000.033.11182822
TGGTCGT5650.032.9805251
GTACTAG6200.032.903441
CTGGTCG5800.032.72252750
ATCGTCA5100.031.82245610
GCTCGAT6100.030.53720364
TCGTCTA6300.029.56958454
TCGATAA6150.029.16532966
TCAGCGC6750.028.10417644
GTCGTCT6750.028.10417653
CGTCTAC6900.027.49912855
CCGTCGG3900.027.4163124
ACCGTCG3900.027.4163123
ACGAGTC6050.027.36514726
GCTCGTC6950.027.29542439
GTCAGCG7100.026.7187643
Produced by FastQC (version 0.11.5)