FastQCFastQC Report
Thu 26 May 2016
ERR1357010_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1357010_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251871
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG2500.031.73732423
CGATAAT5800.028.55185567
CTCGATA5800.027.95578265
ATGCGAC5600.027.72087721
GGTATCA34950.027.5610981
TCGTCTA6050.026.80713354
CAATGCG5850.026.53622419
TGCGACG5800.026.17020822
TGGTCGT6500.024.950751
CGTACGA2800.024.64023260
TCGATAA6600.024.56829366
CGTCTAC6750.024.5383555
GCTCGAT6700.024.1999964
GTACTAG8850.023.797211
CCGTCGG3200.023.7167524
CGTCATT7150.023.64031426
ACGAGTC6450.023.53185526
GTATCAA52850.023.5178181
CTAACGC3100.023.3882163
CATTGCG7100.023.32042129
Produced by FastQC (version 0.11.5)